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Gas Chromatography-Mass Spectrometry and High Performance Liquid Chromatography Analyses of Persea Americana (Lauraceae) Seeds Extract
Proceedings of International Conference on Life Sciences, Engineering and Technology - 2022
Victoria N. Umeh, Ubachukwu I. Olli, Peter A. Akah, Jude H. Okoyeh
Dr. Catherina Zeman, Dr. Hakan Akcay
978-1-952092-32-9
50-57
Plants have been found useful in the treatment of various diseases affecting man. Several pharmacological activities of aqueous seed extract of Persea Americana (Avocado) (ASEPA) has been reported. Pharmacological investigations had attributed strong anti-ulcer activity to ASEPA. High performance liquid chromatography (HPLC) for its major compounds and the GC-MS analysis (gas chromatography-mass spectrometry) for structure elucidation of compounds in the seed extract is yet to be carried out. Knowledge of the major compounds might guide scientists to a novel anti-ulcer drug. The aim of the study was to carry out high performance liquid chromatography (HPLC) and GC-MS study on the aqueous seed extract of P. Americana (ASEPA) and fractions. Dried and pulverized seeds of P. americana was soaked in distilled water (25 oC) for 24 hours, filtered and freeze-dried. The extract was fractionated with n-hexane and residue was the water fraction. Analytical high performance liquid chromatography (HPLC) was used for identification of the major compounds. HPLC was able to identify polar compound. sample from VLC analysis was used for GC-MS analysis using Shimdzu (GCMS-QP2010plus) system in order to identify non-polar compounds in the extract and fractions. HPLC analysis revealed major polar compound as Abscisic acid while GC-MS analysis elucidated structures of 24 monosaturated fatty acids. P. americana seeds extract and fraction contain mainly Abscisic acid and long-chain monosaturated fatty acids
Umeh, V.N., Olli, U.I., Akah, P.A., & Okoyeh, J.H. (2022). Gas Chromatography-Mass Spectrometry and High Performance Liquid Chromatography Analyses of Persea Americana (Lauraceae) Seeds Extract. In C.L. Zeman & H. Akcay (Eds.), Proceedings of ILSET 2022-- International Conference on Life Sciences, Engineering and Technology (pp. 50-57), Los Angeles, USA. ISTES Organization.
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