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Ex vivo Nitrate Exposure, DNA Damage Indicators, and Cytokine Response of Peripheral Blood Mononuclear Cells
Proceedings of International Conference on Life Sciences, Engineering and Technology - 2022
Junu Shrestha, Lisa Beltz, Catherine L. Zeman
Dr. Catherina Zeman, Dr. Hakan Akcay
978-1-952092-32-9
Nitrogen in the form of NO is a critical biomolecule (Halliwell & Gutteridge, 2007). However, when excessive amounts of agriculturally derived, soluble nitrogen contaminate drinking water supplies and reach high concentrations in human body fluids, reactive nitrogen can create both acute and chronic health concerns. This study examined lymphocytes in-vitro, examining varying exposures of nitrite (in the form of sodium nitrite at 1, 5, 10, 15 ppm) and lymphocyte reactions to these exposures from the perspective of inflammatory cytokine expression and indicators of DNA stress and damage such as evidence of induction pathway dephosphorylation and histone indicators of DNA strand breakage. This study illustrated that IL-1, IL-6, IL-17, TNF-
Shrestha, J., Beltz, L., & Zeman, C.L. (2022). Ex vivo Nitrate Exposure, DNA Damage Indicators, and Cytokine Response of Peripheral Blood Mononuclear Cells. In C.L. Zeman & H. Akcay (Eds.), Proceedings of ILSET 2022-- International Conference on Life Sciences, Engineering and Technology (pp. 1-21), Los Angeles, USA. ISTES Organization.
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